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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Guanine nucleotide-binding protein G (I)/G (S)/G (O) subunit gamma-12 (Gng12)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Guanine nucleotide-binding protein G (I)/G (S)/G (O) subunit gamma-12 (Gng12)品名简称:
Recombinant Mouse Gng12 protein货号:
CSB-EP866734MO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Gng12Uniprot No.:
Q9DAS9别名:
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
15.0 kDa表达区域:
2-69aa氨基酸序列:
SSKTASTNSIAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSDPLLMGIPTSENPFKDKKTC蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.功能2:
/文献:
"Disruption of G-protein gamma5 subtype causes embryonic lethality in mice." Moon A.M., Stauffer A.M., Schwindinger W.F., Sheridan K., Firment A., Robishaw J.D. PLoS One 9:e90970-e90970(2014)研究领域:
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文献和实验Nucleotide Binding/Hydrolysis Assay
2 DDW Sephadex G-50 Medium column (0.8 cm in x 20 cm) Column buffer = 50 mM Tris-OAc, pH 7.5 1 mM EGTA 1 mM MgCl2 Scintillation fluid
Finding Protein and Nucleotide Similarities with FASTA
for the programs and 100 to 600 Mb of disk space for protein sequence databases. The FASTA programs require very little memory over that required by the computer's operating system. Software
[35S]GTPS Binding as an Index of Total G-Protein and G-Subtype-Specific Activation by GPCRs
binding step, Gα-subunit-specific antibodies can be used to investigate the Gα-protein subpopulations activated by receptors by immunoprecipitation of [35 S]GTPγS–Gα complexes and quantification by scintillation counting. Here, we describe a total [35 S
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