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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse 3-ketoacyl-CoA thiolase A, peroxisomal (Acaa1a)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse 3-ketoacyl-CoA thiolase A, peroxisomal (Acaa1a)品名简称:
Recombinant Mouse Acaa1a protein货号:
CSB-EP852843MO规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Acaa1aUniprot No.:
Q921H8别名:
Acetyl-CoA C-myristoyltransferase;Acetyl-CoA acyltransferase A;Beta-ketothiolase A;Peroxisomal 3-oxoacyl-CoA thiolase A种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
48.2 kDa表达区域:
27-424aa氨基酸序列:
SARFPQASASDVVVVHGRRTPIGRASRGGFKNTTPDELLSAVLTAVLQDVRLKPEQLGDISVGNVLEPGAGAVMARIAQFLSGIPETVPLSTVNRQCSSGLQAVANIAGGIRNGSYDIGMACGVESMSLSGMGNPGNISSRLLESEKARDCLTPMGMTSENVAERFGISRQKQDDFALASQQKAASAQSRGCFRAEIVPVTTTVLDDKGDKKTITVSQDEGVRPSTTMQGLAKLKPAFKDGGSTTAGNSSQVSDGAAAVLLARRSKAEELGLPILGVLRSYAVVGVPPDVMGIGPAYAIPAALQKAGLTVNDIDIFEINEAFASQAVYCVEKLGIPAEKVNPLGGAIALGHPLGCTGARQVVTLLNELKRRGRRAYGVVSMCIGTGMGAAAVFEYPGN蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Responsible for the thiolytic cleavage of straight chain 3-keto fatty acyl-CoAs (3-oxoacyl-CoAs) (Probable). Plays an important role in fatty acid peroxisomal beta-oxidation (Probable). Catalyzes the cleavage of short, medium, long, and very long straight chain 3-oxoacyl-CoAs功能2:
/文献:
/研究领域:
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文献和实验三句话读懂一篇 CNS:基因评分挑选聪明小孩之争议;高学历女性更瘦、男性更胖……
海洛 plus6. Nat Metab:揭示醋酸调控细胞衰老的新机制目前,对于醋酸的研究层出不穷。2021 年 6 月 28 日,湖北大学李珊珊研究团队在 Nature Metabolism 上发表研究论文 The SESAME complex regulates cell senescence through the generation of acetyl-CoA。该研究发现醋酸促进端粒附近区域组蛋白 H4K16 乙酰化水平,揭示其加速细胞衰老的分子机制。图 6:来源 Nature
2: Monitoring CoA Release using a DTNB‐coupled Assay to Determine N‐Acylasetransferase Activity Alternate Protocol 5: Direct Assay of N‐Acyltransferase Activity by Monitoring the Disappearance of Thioester Bond Absorbance
-elongation assay used 4 µM of [2-14C]malonyl-CoA (60 mCi/mmol) and 20 µM of acetyl-CoA as substrates. The reaction was carried out at 37°C for 30 min with or without an inhibitor, directly applied to a l6% polyacrylamide gel containing 0.5 M urea
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