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小鼠Wnt9a重组蛋白Protein Wnt-14Mouse

Protein Wnt-9a(Wnt9a)蛋白
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  • ¥1836
  • CUSABIO已认证
  • CN
  • CSB-EP840305MO
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Mouse Protein Wnt-9a (Wnt9a)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Mouse Protein Wnt-9a (Wnt9a)

    品名简称:

    Recombinant Mouse Wnt9a protein

    货号:

    CSB-EP840305MO

    规格:

    20μg

    价格:

    1836

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    Wnt9a

    Uniprot No.:

    Q8R5M2

    别名:

    Protein Wnt-14

    种属:

    Mus musculus (Mouse)

    蛋白长度:

    Full Length of Mature Protein

    来源:

    E.coli

    分子量:

    44.3 kDa

    表达区域:

    30-365aa

    氨基酸序列:

    YFGLTGSEPLTILPLTLETEAAAQAHYKACDRLKLERKQRRMCRRDPGVAETLVEAVSMSALECQYQFRFERWNCTLEGRYRASLLKRGFKETAFLYAISSAGLTHALAKACSAGRMERCTCDEAPDLENREAWQWGGCGDNLKYSSKFVKEFLGRRSSKDLRARVDFHNNLVGVKVIKAGVETTCKCHGVSGSCTVRTCWRQLAPFHEVGKHLKHKYETSLKVGSTTNEATGEAGAISPPRGRASGSGGGDPLPRTPELVHLDDSPSFCLAGRFSPGTAGRRCHREKNCESICCGRGHNTQSRVVTRPCQCQVRWCCYVECRQCTQREEVYTCKG

    蛋白标签:

    C-terminal 6xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    13-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity .

    功能2:

    /

    文献:

    /

    研究领域:

    Cancer

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    相关实验
    • v468 Chapter 2 Wnt活性蛋白分离及应用

      activity of the Wnt protein. 9. Mouse L cells lack cadherins and consequently have very littleto no membrane-associated β-catenin. As a result, the Wntstimulatedcytoplasmic accumulation of β-catenin is readilydetectable. If cells with cadherins are used

    • v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性

      multiplerounds of freeze–thawing. Pre-incubation of Wnt samples with sFRP protein for 30 min at 37°C is sufficientto inhibit Wnt activity in various assays. The concentration of sFRP required to block Wnt signalingshould be determined empirically, as it will vary

    • 【原创】Protein A Sepharose 之进化篇。。。

      洗脱条件比较剧烈,最后收集的蛋白质很有可能变性,或者是复性困难。 这种洗脱条件剧烈的Protein A 柱结合的重组蛋白质A一般具有5个串联结构域:E、D、A、B、C。这种重组蛋白A虽然除去了不重要的非结合域,仅由5个结构域组成,每个域均可以和IgG的Fc段结合,但不同的域结合强度略有差异。因此洗脱条件不均一,而且经常需要较低的pH值。如果减少串联结构域的个数,并且采取同型结构域串联,就可以避免不同结构域与抗体Fc 段亲和性的差异,洗脱条件会温和而均一。为此,我们可以做一个试验,比较一下

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    暂无技术资料 索取技术资料

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    小鼠Wnt9a重组蛋白Protein Wnt-14Mouse Protein Wnt-9a(Wnt9a)蛋白
    ¥1836