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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Immunohistochemisty-Fluorescence Protocol
temperature with 15 µL of flurochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1 or IgG2A or IgG2B diluted 1:300) in Wash Buffer-Saponin supplemented with 5 human AB serum. Wash slides three times in Wash Buffer-Saponin and air
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
antibody(1 mg/ml; Chemicon, cat. no. MAB4401) R‐phycoerythrin goat anti‐mouse IgG1 conjugate (1 mg/ml; Invitrogen, cat. no. P21129)
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