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文献和实验High-Level Baculoviral Expression of Hormone-Sensitive Lipase
The limited availability of purified hormone-sensitive lipase (HSL) has been one of the major obstacles in working with this enzyme. The low tissue abundance (approx 1 �g HSL/g adipose tissue) and the labile and hydrophobic character of HSL
Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification
IgM. However, in addition to the complexity of MBP isolation, functional binding capacities of MBP columns are limited to 1 or 2 mg of IgM per milliliter of support. IgA, which is involved in the first specific defense against natural infection
of isolation of nucleoli of the Walker carcinosarcoma and liver of the rat. Cancer Res. 1963; 25:693-697). 1. Seed HeLa cells (ATCC number: CCL-2) on to 10x14 cm Petri dishes and culture at 37oC in 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM
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