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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
We have been karyotyping animals in the Grainger lab to confirm that the stocks maintained in the lab are indeed X. tropicalis and to establish that wild-caught animals being used for gynogenesis are also X. tropicalis . We recently
性,因此在Immunostaining时常使用含 0.1%-0.5%Triton X-100的PBS或TBS。 5. Triton X-100非常稳定,密封避光 +2 to +8℃条件下可以长期保存。 6.Triton X-100是透明,略显粘稠,稍微发黄的液体。 7. Triton X-100的密度为1.07 g/ml,pH6.0-8.0(5%的溶液)。 8. Triton X-100可以高压灭菌。 9. 用Triton X-100来裂解细胞时
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