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RNF182/RNF182蛋白/RING finger pr

otein 182;RING-type E3 ubiquitin transferase RNF182蛋白/Recombinant Human E3 ubiquitin-protein ligase RNF182 (RNF182), partial重组蛋白
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  • ¥69 - 699
  • CUSABIO已认证
  • CN
  • CSB-EP822714HU1
  • 2025年07月08日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 保质期

      12个月

    • 英文名

      Recombinant Human E3 ubiquitin-protein ligase RNF182 (RNF182), partial

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      10ug/20ug/50ug/100ug/200ug/500ug/1mg

    规格:10ug产品价格:¥69.0
    规格:20ug产品价格:¥139.0
    规格:50ug产品价格:¥209.0
    规格:100ug产品价格:¥349.0
    规格:200ug产品价格:¥419.0
    规格:500ug产品价格:¥559.0
    规格:1mg产品价格:¥699.0

    Research Areas

    Cell Biology

    Uniprot ID

    Q8N6D2

    Gene Names

    RNF182

    Alternative Name(s)

    RING finger protein 182;RING-type E3 ubiquitin transferase RNF182

    Lead Time

    3-7 business days

    Organism

    Homo sapiens (Human)

    Source

    E.coli

    Expression Region

    1-183aa

    Protein Length

    Partial

    Tag Info

    C-terminal 6xHis-tagged

    Target Protein Sequence

    MASQPPEDTAESQASDELECKICYNRYNLKQRKPKVLECCHRVCAKCLYKIIDFGDSPQGVIVCPFCRFETCLPDDEVSSLPDDNNILVNLTCGGKGKKCLPENPTELLLTPKRLASLVSPSHTSSNCLVITIMEVQRESSPSLSSTPVVEFYRPASFDSVTTVSHNWTVWNCTSLLFQTSIR

    MW

    27.3 kDa

    Purity

    Greater than 95% as determined by SDS-PAGE.

    Form

    Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid form

    Buffer

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Notes

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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    相关实验
    • Assays for RING Family Ubiquitin Ligases

      classes of E3 ligases, the HECT domain and the RING finger-containing ligases. RING finger-based ubiquitination utilizes RING-containing protein subunits, or proteins with intrinsic RING domains, to catalyze the formation of polyubiquitin chains

    • Studies of the Ubiquitin Proteasome System

      and Translation Systems In Vitro Production of UbiquitinProtein Ligases (E3s) Basic Protocol 24: Expression of RingFinger E3s in Bacterial Lysates Alternate Protocol 14: Expression of HECT and U‐Box E3s in Bacterial Lysates Alternate Protocol 15: Expression

    • In Vitro Protein Ubiquitination Assay

      formation of E2s and Ring/U-box-type E3s, and E3-mediated substrate ubiquitination. E2/E3 specificities can also be investigated quickly by using this system. This method can be applied to ubiquitination assays of proteins from any eukaryotic organisms.

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    文献支持
    RNF182/RNF182蛋白/RING finger protein 182;RING-type E3 ubiquitin transferase RNF182蛋白/Recombinant Human E3 ubiquitin-protein ligase RNF182 (RNF182), partial重组蛋白
    ¥69 - 699