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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Dermatophagoides farinae Mite allergen Der f 6 (DERF6)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Dermatophagoides farinae Mite allergen Der f 6 (DERF6)品名简称:
Recombinant Dermatophagoides farinae DERF6 protein货号:
CSB-EP343783DCO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
DERF6Uniprot No.:
P49276别名:
(Allergen Der f VI)(DF5)(allergen Der f 6)种属:
Dermatophagoides farinae (American house dust mite)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
32.5 kDa表达区域:
50-279aa氨基酸序列:
VVGGQDADLAEAPFQISLLKDYLIMKRHMCGGSLISESTVVTAAHCTYGQKASSLSVRYGTNQRTSSSYGDLKVKPIIQHESYEQDQTQTDKTIIILPNPVVPSTNVQMNEIETEDIVDGDKVTIYGWGLTDGNGKDLPDKLQKGSMTIVGNDRCNEKWGSINAIHPGMICALDKTQSGCNGDSGGPLVSANRKLTGIVSWGPSKCPPGEYMSVFTRPKYYLDWITKNIV蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Protease that shows specificity similar to chymotrypsin.功能2:
/文献:
"Cloning and expression of Der f 6, a serine protease allergen from the house dust mite, Dermatophagoides farinae." Kawamoto S., Mizuguchi Y., Morimoto K., Aki T., Shigeta S., Yasueda H., Wada T., Suzuki O., Jyo T., Ono K. Biochim. Biophys. Acta 1454:201-207(1999)研究领域:
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文献和实验尘螨变应原及酶性变应原的类型及理化、免疫学特性 尘螨 主要存在于人类居住的环境中。能引起人类过敏性疾病的主要螨种有4类:户尘螨(Dermatophagoides pterongssinus,Der p)、粉尘螨(Dermatophagoides farznae,Derf)、微角尘螨(Dermatophagoides mzcroceras,Der m)和埋内欧尘螨(Euroglyphus maynei,Eurm)。在我国以及世界大部分地区,户尘螨和粉尘螨分布
a look from the following website just by clicking "Tyrosine Phosphorylation and Dimerization" http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-43F85YV-2&_user=1111158&_coverDate=06%2F29%2F2001&_rdoc=1&_fmt=full&_orig=search
杆菌的包涵体可采用离心收获。 洗涤 包涵体常用的洗涤试剂为中性去垢剂和还原剂。包涵体中主要由重组蛋白组成,同时也含有一些外膜蛋白(Omp C、Omp F、Omp A)、16S 和 23S、5S的r RNA、质粒 DNA 和其它杂质。去除这些杂质,可采用去垢剂 Triton X-100、脱氧胆酸盐和低浓度的变性剂等对包涵体充分洗涤。包涵体洗涤的洁净程度,对于后续的纯化步骤十分重要,包涵体纯度高,可以大大降低后续的纯化压力。但是,变性剂尿素的使用应该注意的是大肠杆菌外膜蛋白Omp T(37 kD
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