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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Triticum aestivum Ubiquitin-activating enzyme E1 3 (UBA3), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Triticum aestivum Ubiquitin-activating enzyme E1 3 (UBA3), partial品名简称:
Recombinant Triticum aestivum UBA3 protein, partial货号:
CSB-EP333591TQN规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
UBA3Uniprot No.:
P31252别名:
/种属:
Triticum aestivum (Wheat)蛋白长度:
Partial来源:
E.coli分子量:
38.7 kDa表达区域:
429-639aa氨基酸序列:
ESLPTYPLEPQDLKPSNNRYDAQVSVFGSKLQKKMEEANTFVVGSGALGCEFLKNLALMGVSCSSKGKLTITDDDIIEKSNLSRQFLFRDWNIGQAKSTVAATAASAINPSLHIDALQNRACPDTENVFHDTFWEGLDVVINALDNVNARMYMDMRCLYFQKPLLESGTLGAKCNIQMVIPHLTENYGASRDPPEKQAPMCTVHSFPHNID蛋白标签:
N-terminal 6xHis-KSI-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.功能2:
/文献:
"Multiple forms of ubiquitin-activating enzyme E1 from wheat. Identification of an essential cysteine by in vitro mutagenesis." Hatfield P.M., Vierstra R.D. J. Biol. Chem. 267:14799-14803(1992)研究领域:
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文献和实验Purification of E1 and E1-Like Enzymes
Ubiquitin-activating enzyme is the archetype for a family of enzymes catalyzing the ATP-coupled activation of ubiquitin and other class 1 ubiquitin-like proteins required for their subsequent conjugation to cellular targets. The general
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠泛素蛋白 (Ubiquitin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Ubiquitin 单抗包被于酶标板上,标准品和样品中的 Ubiquitin 与单抗结合,加入生物素化的抗大
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