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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Putative ATP-dependent RNA helicase Pl10 (D1Pas1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Putative ATP-dependent RNA helicase Pl10 (D1Pas1)品名简称:
Recombinant Mouse D1Pas1 protein货号:
CSB-EP325821MO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
D1Pas1Uniprot No.:
P16381别名:
/种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
79.9 kDa表达区域:
2-660aa氨基酸序列:
SHVAEEDELGLDQQLAGLDLTSRDSQSGGSTASKGRYIPPHLRNREAAKAFYDKDGSRWSKDKDAYSSFGSRSDTRAKSSFFSDRGGSGSRGRFDERGRSDYESVGSRGGRSGFGKFERGGNSRWCDKADEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALRAMKENGKYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEEADKRSFLLDLLNATGKDSLILVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAFEHHYKGGSRGRSKSRFSGGFGARDYRQSSGASSSSFSSGRASNSRSGGGSHGSSRGFGGGSYGGFYNSDGYGGNYSSQGVDWWGN蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Putative ATP-dependent RNA helicase. Possible role in a key step of the spermatogenic process.功能2:
/文献:
/研究领域:
Cancer请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验的 ULTRAhyb-Oligo 缓冲液(17x 缓冲液)。使用前需在 68℃ 环境中进行溶解。 ( 7 ) 用 0.5 U T4 多核苷酸激酶(PNK,Roche) 和 6 μl [γ-32P ] ATP ( 5000 Ci/mmol ) 标记 1 μmol/L DNA 寡核苷酸(约含有 10 个与目的基因互补的 DNA 寡核苷酸)。 ( 8 ) 参照 Hamilton 和 Baulcombe 的方法,在 35°C 条件下进行 RNA 印迹杂交[14]。 3. 注释 注
快速老化痴呆模型小白鼠SAMP8, SAMP10老化特征研究进展
快速老化模型小白鼠(Senescence Accelerated Mouse,SAM)由日本京都大学竹田俊男教授经20年近代延交培育而成,具备均一的遗传背景和稳定老化病态特征。SAM品系中,P8和P10除具有P品系的一般老化症状外,伴快速老化自然出现学习记忆力障碍和低恐怖低紧张状态,为脑老化痴呆模型小白鼠;其共同点是均具有认知障碍,且在渡过生长期后(4~6月),伴快速老化自然发生,与临床痴呆的渐进性发生极为相似。另外两者还各具特点,病理变化具有一定的差异。P8脑中主要出现明显的β-淀粉样蛋白
机制已日渐清晰。Zamore 等利用果蝇胚胎提取物建立的体外系统, 证实RNAi 是一个依赖ATP 的过程, 在此过程中, dsRNA (外源的或体内产生的) 首先被降解为具5"2单磷酸、长21~23bp 的小分子双链RNA , 这种RNA 分子称为小干扰RNA , siRNA 通过碱基互补配对识别具同源序列的mRNA , 并介导其降解。研究表明在生物体中siRNA 具相似的结构特征: 为长约21~23bp 的双链RNA , 具5"单磷酸和3"羟基末端, 互补双链的3"端均有一个2~3nt 的单链突出
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