手机验证
大量
HiPerFect Transfection Reagent
2年
科邦兴业(北京)科技有限公司
4 ℃保存
kit
HiPerFect Transfection Reagent is a unique blend of cationic and neutral lipids that enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations. In addition to siRNA, HiPerFect Transfection Reagent is ideally suited to transfection of miRNA mimics or inhibitors. Cell-type-specific protocols using HiPerFect Transfection Reagent are available at the TransFect Protocol Database.
真核细胞的siRNA和miRNA转染
低浓度条件下的siRNA高效转染
高效转染原代细胞,细胞存活率高
高效转染悬浮细胞和巨噬细胞
TransFect Protocol Database中有针对特殊细胞类型的实验方案
高效转染miRNA模拟物或抑制剂
HiPerFect Transfection Reagent是一种阳离子和中性脂组成的独特混合物,能够有效摄入siRNA和有效释放细胞内部的siRNA,即使使用低浓度的siRNA也可高效敲除基因。除了siRNA,HiPerFect Transfection Reagent还适用于低通量和高通量的转染miRNA模拟物或抑制剂。TransFect Protocol Database中有适用于特殊细胞类型的实验方案。
Transfection of siRNA can result in off-target effects, in which siRNAs affect the expression of non-homologous or partially homologous gene targets. Off-target effects, which may produce misleading results in RNAi experiments, can be largely avoided by using low siRNA concentrations. Using HiPerFect Transfection Reagent, highly efficient transfection and silencing have been observed, in some cases with as little as 10 pM siRNA. HiPerFect Transfection Reagent provides highly efficient siRNA transfection over a range of siRNA concentrations from low to high, allowing researchers to choose the siRNA concentration they wish to use. It can be used to transfect a wide range of cell types, including HeLa, HeLa S3, HEK 293, NIH/3T3, Huh-7, HepG2, MCF-7, HUVEC, and NHLF (see figure "Efficient transfection of NHEK").
microRNAs (miRNAs) are a class of endogenous small RNA molecules with similar characteristics to siRNAs. miRNAs play a role in many diverse biological processes such as development, differentiation, and apoptosis. Transfection of synthetic miRNA mimics or inhibitors is a technique used to elucidate the targets and roles of particular miRNAs. miRNA mimics are chemically synthesized miRNAs which mimic naturally ocurring miRNAs upon transfection into the cell. miRNA inhibitors are single-stranded modified RNAs which specifically inhibit miRNA function. Reduced gene expression after transfection of an miRNA mimic, or increased expression after transfection of an miRNA inhibitor, provide evidence that the targeted miRNA is involved in regulation of that gene. Alternatively, the role of miRNAs in various pathways can be studied by examination of a specific phenotype after transfection of an miRNA mimic or inhibitor. HiPerFect Transfection Reagent was developed for highly efficient transfection of eukaryotic cells with siRNA, and is ideally suited to transfection of miRNA mimics or inhibitors.
HiPerFect Transfection Reagent is provided as a ready-to-use solution - just add the reagent to your diluted siRNA/miRNA, mix, incubate, and pipet the complexes onto the cells. Transfections can be performed in the presence of serum, eliminating the need to remove complexes from the cells. In addition to the protocols provided in the HiPerFect Transfection Reagent Handbook, you can find protocols to suit your cell type and plate/dish format using the TransFect Protocol Database. The database takes the guesswork out of transfection protocols. Rather than adapting existing protocols to fit your requirements, the database provides exactly the protocol needed, saving time and effort. Simply enter the cell type, nucleic acid, and plate format to receive a QIAGEN transfection protocol to print out or download in convenient PDF format. Use of the TransFect Protocol Database is free of charge and no registration is required.
Transfection with HiPerFect Transfection Reagent can be carried out using the common transfection procedure of seeding the cells 24 hours before transfection. Alternatively, protocols are provided for reverse transfection in 96-well plates and 384-well plates (see the HiPerFect Transfection Reagent Handbook). In these protocols, cells are seeded and transfected in the same day. siRNA/miRNA is spotted into wells followed by the addition of HiPerFect Reagent. After complex formation, cells are added to the wells (see flowchart " Reverse transfection using HiPerFect Transfection Reagent"). Reverse transfection protocols are rapid, convenient, and can easily be automated.
HiPerFect Transfection Reagent enables highly efficient siRNA transfection, even with low siRNA concentrations, for applications such as:
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
资料下载:
301705EN-HiPerFect-Transfection-Reagent-Handbook.pdf 附件下载 (下载 0 次)
301705_169037180_en_1042.pdf 附件下载 (下载 0 次)
请 [登录] 后再下载!
需要更多技术资料 索取更多技术资料
301705_169037180_en_1042.pdf 附 (下载 0 次)