Lire1/Lire1蛋白Recombinant Mouse

纯度：

Greater than 90% as determined by SDS-PAGE.

基因名：

Lire1

Uniprot No.：

P11260

别名：

(L1-ORF1p)(LINE retrotransposable element 1)(LINE1 retrotransposable element 1)(Transposase element L1Md-A101/L1Md-A102/L1Md-A2)

种属：

Mus musculus (Mouse)

蛋白长度：

Full Length

来源：

E.coli

分子量：

48.7 kDa

表达区域：

1-357aa

氨基酸序列：

MAKGKRKNPTNRNQDHSPSSERSTPTPPSPGHPNTTENLDPDLKTFLMMMIEDIKKDFHKSLKDLQESTAKELQALKEKQENTAKQVMEMNKTILELKGEVDTIKKTQSEATLEIETLGKRSGTIDASISNRIQEMEERISGAEDSIENIDTTVKENTKCKRILTQNIQVIQDTMRRPNLRIIGIDENEDFQLKGPANIFNKIIEENFPNIKKEMPMIIQEAYRTPNRLDQKRNSSRHIIIRTTNALNKDRILKAVREKGQVTYKGRPIRITPDFSPETMKARRAWTDVIQTLREHKCQPRLLYPAKLSITIDGETKVFHDKTKFTQYLSTNPALQRIITEKKQYKDGNHALEQPRK

蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged

产品提供形式：

Liquid or Lyophilized powder

缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

货期：

3-7 business days

注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

功能：

Nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. Functions as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded.

内毒素：

Not test.

SDS-PAGE:

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

LC-MS Image Description/Western Blot:

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Product types:

In Stock Protein

Biological_Activity:

/

Research Areas:

Others

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Reference:

"Mobilization of LINE-1 retrotransposons is restricted by Tex19.1 in mouse embryonic stem cells." MacLennan M., Garcia-Canadas M., Reichmann J., Khazina E., Wagner G., Playfoot C.J., Salvador-Palomeque C., Mann A.R., Peressini P., Sanchez L., Dobie K., Read D., Hung C.C., Eskeland R., Meehan R.R., Weichenrieder O., Garcia-Perez J.L., Adams I.R. Elife 6:0-0(2017)

Function:

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