dnaK|dnaK蛋白|dnaK蛋白|dnaK pyogen

Heat-Shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli

of scaffolding effects and ATP-driven conformational changes that mediate the temporary unfolding and subsequent refolding of protein substrates. DnaK and GroEL are the two major Escherichia coli chaperones. They belong to the HSP70 and HSP60 families of proteins

Inhibition ofChaperone-Dependent Bacterial Ribosome Biogenesis

to all of the other DnaK-dependent cellular functions. An easily testable phenotype of DnaK is described here based on α-complementation of β-galactosidase. This protein fragment complementation requires a functional DnaK in vivo , offering a suitable method for screening

Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response

, which are the signal initiating the response. The heat-shock response, therefore, functions to maintam the proteinacious component of the cell in an active form. Thus, by inducing heat-shock protein synthesis, the cell is better able to survive the stress condition