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文献和实验Non-fluorescent protein/RNA double-labeling
the embryos with PBT:Hybridization buffer (1:1), and then with hybridization buffer alone. Hybridization buffer for DNA probes is: 5 X SSC, 100 ug/ml sonicated, boiled salmon sperm DNA, 100 ug/ml tRNA, 50 ug/ml heparin, and 0.1% Tween-20 (for RNA probes
by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse IgG1 heavy chain do stain rat IgG2a and IgG2b . Procedure: First stain HRP Developing solution Second stain AP
in the same species. However better results are obtained if two different species are used. Careful check possible crossreactivty of your secondary antibodies by staining in simple IHC the relevant heterologous primary antibodies. E.g. many goat anti mouse
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