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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
优利科(上海)生命科学有限公司
- 库存:
55
- 靶点:
/
- 级别:
科研
- 目录编号:
KT0895
- 克隆性:
多克隆
- 抗原来源:
/
- 保质期:
1年
- 抗体英文名:
APNG/MPG
- 抗体名:
Rabbit Anti-APNG/MPG antibody
- 标记物:
详询
- 宿主:
兔
- 适应物种:
Human, (predicted: Mouse, Rat, Pig, Cow, )
- 免疫原:
KLH conjugated synthetic peptide derived from human APNG/MPG: 101-200/298
- 亚型:
IgG
- 形态:
Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 保存条件:
-20℃
- 浓度:
1mg/ml
- 规格:
50ul
中文名称:N-甲基嘌呤DNA糖基化酶
英文名称:APNG/MPG
别 名:MPG; N-methylpurine-DNA glycosylase isoform a; DNA 3 methyladenine glycosylase; 3 alkyladenine DNA glycosylase; AAG; ADPG; Alkyladenine DNA glycosylase; anpg ; APNG; MDG; N methylpurine DNA glycosirase; CRA36.1; MDG; Mid1; PIG11; PIG16; 3MG_HUMAN.
货号:YLK-KT0895
分 子 量:33kDa
细胞定位:细胞核
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Function:
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Subunit:
Belongs to the DNA glycosylase MPG family.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the DNA glycosylase MPG family.
SWISS:
P29372

注意:
所提供的本产品仅供研究使用,不得用于人类、治疗或诊断应用。


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文献和实验Synthesis of [15N]-Labeled DNA Fragments
DNA fragments labeled with 15 N have the potential to provide novel insight into base pairing, hydration, drug/nucleic acid, and protein/ nucleic acid interactions. Synthetic routes to a variety of [15 N]-labeled pyrimidine nucleosides (1 –
Syntheses of DNA Duplexes That Contain a N4C‐Alkyl‐N4C Interstrand Cross‐Link
. and Miller, P.S. 2006. Formation and repair of interstrand cross‐links in DNA. Chem. Rev. 106:277‐301. Noronha, A.M., Noll, D.M., Wilds, C.J., and Miller, P.S. 2002a. N(4)C‐ethyl‐N
」和「扩增区」分割开 PCR 体系配置和 PCR 扩增实验。如果不具备这样的条件,首先要避免荧光定量 PCR 实验结束之后开盖,以防扩增产物形成气溶胶对后续实验造成污染。少量意外开盖不能避免时,可以使用含有 UNG 酶(uracil-N-glycoslyase, 尿嘧啶 DNA 糖基化酶)的扩增预混液。如果在 PCR 反应中以 dUTP 代替 dTTP 参入到 PCR 产物中,形成了含有 dUTP 的扩增产物,UNG 酶能选择性断裂单链和双链 DNA 中 U 碱基的糖苷键, 降解 PCR 扩增










