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文献和实验and alternate complement pathway, and serves as an amplification step C3b (activated C3) can amplify by cleaving more C3 via the alternate complement pathway Positive staining (normal): follicular dendritic cells, granulocytes
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Purification and Characterization of Human and Mouse Complement C3
Complement component C3 is the most abundant complement protein in plasma, central to all three complement activation pathways and essential to complement amplification. Thus, it is one of the most extensively studied complement proteins
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