Flex-T™ Biotin H-2 D(b) Flu Mo

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

to avidin magnetic beads (Dynal™). The final elutes, following the binding of the biotin linked RNAs with the avidin magnetic beads and washing, were then DNAse, RNAse H or RNAse A treated, RT (BioRad™ iScript) reacted and PCR amplified. References 1.Kim

Use of Biotin-Labeled Probes on Plant Chromosomes

away from bright light. Alternative methods of probing and detection are given in chapter 26 and chapter 27 . The whole procedure can be run in 1 d, with a 4-h hybridization period, but we prefer to begin the method in the afternoon and to hybridize

在无血清细胞培养条件下将人 iPS 细胞体外分化为结肠类器官

% 才能进行步骤 3。如果 CDX2 的表达低于 60%，则再进行一天HE分化并重新检查 CDX2 的表达。 图 3. 定形内胚层细胞分化为后肠内胚层细胞。后肠内胚层诱导后A) 第2 天 B) 第 3 天和 C) 第 4 天后肠内胚层细胞的形态。 步骤3：结肠类器官的扩增和冷冻保存（第 8-48 天） 结肠类器官的扩增 从培养箱中取出含有第 8 天后肠内胚层培养物的 6 孔板。 从每个孔中吸出培养基。 用 3 mL 1X PBS 清洗每个孔。 将 2 mL 预热的 3dGRO™ 人类结肠