Rat Bone Morphogenetic protein 2 (BMP-2) ELISA Kit (1 plate)
CAS号:
/
保质期:
6个月
供应商:
齐源生物
保存条件:
2-8℃
规格:
1plate/2plates
规格:
1plate
产品价格:
¥7653.8
规格:
2plates
产品价格:
¥13659.8
大鼠骨形态发生蛋白 2 (BMP2)ELISA 测定试剂盒详细如下:
Product Description The rat Bone Morphogenetic protein 2 (BMP-2) Kit is a sandwich ELISA. The capture antibody is a monoclonal BMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known BMP-2 concentration are added to these wells and allowed to complex with the bound BMP-2 antibody. A biotinylated BMP-2 monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat BMP-2 in samples such as culture supernatant, serum and tissue homogenates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application(s) ELISA
Application Details ELISA. For the quantification of Bone morphogenetic protein 2 (BMP2) in Culture Supernatant, Tissue Homogenates, Serum. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Target Bone morphogenetic protein 2 (BMP2)
Specificity Human; Mouse; Rat; The antibodies in this kit cross react with human, rat and mouse BMP-2 and likely other untested species.
Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
Target Host Species Rat
Species Reactivity Rat
Immunogen Description The standard and immunogen in this assays is produced in and purified from a mammalian expression system consisting of amino acids Q283-R396
Range 31.2 pg/mL - 2,000 pg/mL
Sensitivity < 2 pg/mL
Sample Type Culture Supernatant, Serum, Tissue Homogenates
Detection Method Colorimetric
Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
Storage Instructions Store at 2-8°C
Storage Temperature 2-8°C
Batch Number Please see item label.
Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.