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文献和实验. In addition, certain subclasses of rat IgG have been found to interact efficiently with human Fc receptors and the human complement system. More important perhaps in practical terms is that, when grown as an ascites, up to 30 mL of fluid containing 1–5 mg/mL
Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
7.16 anti-rat IgG2b). -------------------------------------------------------------------------------- 5) For full details see: Clark, M.R., & Waldmann, H. Production of murine monoclonal antibodies in Methods in Hematology 13:1-20 (1986
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