Recombinant Human Platelet-derived Growth Factor-AA (Human PDGF-AA)
规格:
10 μg
Product Information
Product Name
Cat#
Size
Price(¥)
Recombinant Human Platelet-derived Growth Factor-AA (Human PDGF-AA)
91601ES10
10 μg
1855.00
91601ES60
100 μg
10215.00
91601ES76
500 μg
26545.00
Product Description
PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely expressed in normal and transformed cells. The mitogenic properties of PDGF AA are well established. FGF-2 and platelet-derived growth factor-A (PDGF-AA) are potent modulators of oligodendrocytes, the main responsible cells for myelin regeneration. PDGF has been suggested to have a significant role in wound healing.
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
>95% by SDS-PAGE analyses.
Biological Activity
The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye. Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.
Endotoxin
< 0.1 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.