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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
有效期1年
- 保存条件:
-20℃至-80℃保存
- 供应商:
翌圣生物科技(上海)股份有限公司
- 库存:
大量
- 英文名:
Recombinant Mouse Granulocyte Colony Stimulating Factor (Mouse G-CSF)
- 规格:
10μg
| Product Name |
Cat# |
Size |
Price(¥) |
| Recombinant Mouse Granulocyte Colony Stimulating Factor (Mouse G-CSF) |
91107ES10 |
10 μg |
1855.00 |
| 91107ES60 |
100 μg |
10215.00 |
|
| 91107ES76 |
500 μg |
26545.00 |
Product Description
Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Human G-CSF is 73% identical at the amino acid level to mouse G-CSF and the two proteins show species cross-reactivity. G-CSF receptor was expressed on cardiomyocytes and G-CSF activated the Jak/Stat pathway in cardiomyocytes. The G-CSF treatment did not affect initial infarct size at 3 d but improved cardiac function as early as 1 week after myocardial infarction. And the G-CSF induces stem cell mobilization by decreasing bone marrow SDF-1 and up-regulating CXCR4.
Product Properties

| Synonyms |
G-CSF, CSF-3, MGI-1G, GM-CSF beta, MGC45931, Pluripoietin |
| Accession |
|
| GeneID |
|
| Source |
E.coli-derived mouse G-CSF protein, Val31-Ala208. |
| Molecular Weight |
Approximately 18.9 kDa. |
| AA Sequence |
VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA |
| Tag |
None |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity |
The ED50 as determined by a cell proliferation assay using mouse NFS-60 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg. Fully biologically active when compared to standard. |
| Endotoxin |
< 0.1 EU per 1μg of the protein by the LAL method. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCl, 0.01% Tween-20. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only!
HB220210
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文献和实验小鼠粒细胞集落刺激因子(G-CSF)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠粒细胞集落刺激因子 (G-CSF)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 G-CSF 单抗包被于酶标板上,标准品和样品中的 G-CSF与单抗结合,加入生物
Hematopoietic Stem Cell Mobilization with G-CSF
stem cells are collected after G-CSF administration. In this chapter we describe peripheral blood stem cell mobilization in autologous transplant patients and in allogeneic donors using G-CSF.
Hematopoietic Stem Cell Mobilization with Agents Other than G-CSF
factor (G-CSF) are the preferred source for transplantation. G-CSF mobilization regimens, however, are associated with known morbidities and a significant number of normal donors and patient populations fail to mobilize sufficient numbers of hematopoietic
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