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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
有效期1年
- 保存条件:
-20℃至-80℃保存
- 供应商:
翌圣生物科技(上海)股份有限公司
- 库存:
大量
- 英文名:
Recombinant Rat Interleukin-4 (Rat IL-4)
- 规格:
5μg
Product Information
| Product Name |
Cat# |
Size |
Price(¥) |
| Recombinant Rat Interleukin-4 (Rat IL-4) |
90169ES08 |
5 μg |
815.00 |
| 90169ES60 |
100 μg |
8515.00 |
|
| 90169ES76 |
500 μg |
22115.00 |
Product Description
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced by by mast cells, activated T cells and bone marrow stromal cells. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively.It is a potent cytokine produced by T cells and to a lesser extent by tumor-associated natural killer cells, basophils, and mast cells. IL-4 is a differentiation factor for transforming growth factor-beta secreting Th3. And IL-4 activates two distinct signalling pathways through tyrosine phosphorylation of Stat6, a signal transducer and activator of transcription, and of a 170K protein called 4PS.
Product Properties

| Synonyms |
Interleukin-4, IL-4, BSF-1, BCDF, IL4E12, Pitrakinra |
| Accession |
|
| GeneID |
|
| Source |
E.coli-derived rat IL-4 protein, His23-Ser147. |
| Molecular Weight |
Approximately 14.0 kDa. |
| AA Sequence |
HGCNDSPLRE IINTLNQVTE KGTPCTEMFV PDVLTATRNT TENELICRAS RVLRKFYFPR DVPPCLKNKS GVLGELRKLC RGVSGLNSLR SCTVNESTLT TLKDFLESLK SILRGKYLQS CTSMS |
| Tag |
None |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat splenocytes is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
| Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
| Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5% trehalose. |
| Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only!
HB220214
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上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠白介素 -4(IL-4)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 IL-4 单抗包被于酶标板上,标准品和样品中的 IL-4 与单抗结合,加入生物素化的抗大鼠 IL-4 ,形成免疫复合物连接在板上,辣根










