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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
6个月
- 英文名:
RNA Broad Range Standard, 100 ng/uL 99850
- 库存:
99
- 供应商:
Biotium
- CAS号:
1
Stand-alone total mammalian RNA standard provided with the AccuBlue® Broad Range RNA Quantitation Kit (31073).
- Mammalian cell total RNA in 1X Tris-EDTA
- 100 ng/uL
- 300 uL
- Store at -20°C or below (can be stored at -80°C for long-term stability)
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文献和实验is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
The gene we are interested in looking at is not very abundant. At 100 ng/ul, the CT value is 30.9 In the literature that I have looked at, people seems to using much lower levels of cDNA. I would actually
The performance of many bacterial genetic experiments would benefit from a convenient method to clone large sets of genes (20–100+kb) and transfer these genes to a wide range of other bacterial recipients. The VEX-Capture technique allows
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
it! Remember ug/KB x 0.66 = picomole thus 1ng of 300bp probe =2 femptomole. GELSHIFT 1)Nuclear extract: see nucprep.ptc & nucext.ptc extracts should be at least 3ug/ul Probe preparation: see endlabl.ptc & isotach.ptc. probe shouldbe 10©20k cpm/ul











