Hot Start Taq DNA Polymerase Cat No: PC1110 Package: 500U/1000U/2500U. Enzyme Activity: 5U/μl Storage:Store at -20℃,valid for one year. Product Description This product is a hot-start Taq DNA Polymerase modified with anti-Taq monoclonal antibody.Anti-Taq monoclonal antibody binds to Taq enzyme before high temperature heating, inhibits polymerase activity, thereby inhibiting non-specific amplification caused by non-specific annealing or primer dimer under low temperature conditions.The anti-Taq monoclonal antibody has been denatured in the initial DNA denaturation step of the PCR reaction, so no special inactivation treatment is required and it can be used under conventional PCR reaction conditions.In the qPCR reaction, the amplification efficiency of the fluorescent PCR can be significantly improved (especially for the low copy number template), and the perfection of the amplification curve can be improved, and it has good stability, high repeatability and strong specificity. At room temperature for one week, the activity of this enzyme can remain above 95%. Taq DNA Polymerase is purified from E.coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. Taq DNA polymerase has 5′to 3′ DNA polymerase activity and 5′to 3′exonuclease activity lacking 3′-5′exonuclease activity. In PCR reaction the extension rate is about 1-2 kb/min. Template-independent “A” can be generated at the 3’ end of the PCR product. It can be used in PCR for TA-Vector cloning, PCR amplification of genomic DNA, DNA sequencing, DNA labeling. Unit DefinitionOne unit (U) is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into an acid-insoluble material in 30 minutes at 74°C, with activated salmon sperm DNA used as template. Quality control Purity of Taq DNA Polymerase detected by SDS-PAGE more than 99% and no exogenous nuclease activity after detection. There is no host remain DNA detect by PCR, it can amplify the single copy genes in Human Genome. There is no enzyme activity change after store at room temperature for one week, Storage buffer: 20mM Tris-HCl (pH 8.0); 0.1mM EDTA; 1mM DTT; 100mM KCl; 50% glycerol; stabilizer. Application It is often used to amplify no more than 6 kb fragments such as PCR, DNA marker, primer extension, sequence determination, flat end plus A. The product can be used for TA-Vector cloning directly. PCR condition The pre-denaturation temperature is 95℃ for 5-10 minutes. The other reaction conditions are the same as the ordinary Taq enzyme. Reaction Components (50μlVolume)