VitroGel ORGANOID Discovery Ki

类器官培养与应用——卵巢癌类器官

Inhibitor Response in Short-Term Patient-Derived Ovarian Cancer Organoids DNA Repair Profiling of HGSC Organoids[J]. Cancer discovery, 2018, 8(11): 1404-1421. 【7】Liu H D. et al. Organoid of ovarian cancer: genomic analysis and drug screening[J]. Clinical

Sequencing Protocol

which is described by McKernan, Blanchard, Kotler and Costa. [1] and Valouev et al [2] and Cloonan et al [3]The advent of DNA sequencing has significantly accelerated biological research and discovery. The rapid speed of sequencing attainable with modern DNA

类器官培养与应用——肾类器官

1 培养基换液，细胞将重新聚合成EB。 6、培养 24h 后，将 EB 转移到 U 型底低吸附的 96 孔板中，加入 Step2 培养基，培养 36h。 7、将细胞用 Step3 培养基换液，培养 48h。 8、将细胞用 Step4 培养基换液，培养 42h。 中肾管（Wolffian duct, WD）前体细胞成熟 9、将上清吸出，加入 0.25% 胰酶/EDTA 至没过细胞，37℃ 消化 6min 解离细胞球。 10、血清封闭后，加入含有 CXCR4/KIT 抗体的稀释液（含 1×HBSS