Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.1%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type
Average(%)
Recovery Range(%)
Serum
98
92-104
Plasma
100
94-106
线性
给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample
1:2
1:4
1:8
1:16
serum(n=5)
95-105%
97-105%
94-104%
105-115%
EDTA plasma(n=5)
105-115%
83-92%
92-102%
112-124%
heparin plasma(n=5)
94-104%
93-105%
102-111%
89-102%
文献
1. Identification and characterization of neurotrophic factors in porcine small intestinal submucosa
Auth: KJ Yang, KC Park, H Choi, et al.
From: Tissue Engineering and Regenerative Medicine