Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.9%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type
Average(%)
Recovery Range(%)
Serum
100
94-106
Plasma
102
96-108
线性
给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample
1:2
1:4
1:8
1:16
serum(n=5)
101-111%
105-116%
103-115%
97-105%
EDTA plasma(n=5)
100-108%
100-109%
108-118%
100-111%
heparin plasma(n=5)
94-104%
107-116%
92-101%
81-91%
文献
1. Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats
Auth: D Li, J Peng, H Xin, et al.
From: Peptides
2. Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor
Auth: Dai Li Ren Guo Qing-Quan Chen Chang-Ping Hu Xiang Chen
From: canadian journal of physiology and pharmacology