Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.8%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type
Average(%)
Recovery Range(%)
Serum
96
90-102
Plasma
98
92-104
线性
给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample
1:2
1:4
1:8
1:16
serum(n=5)
82-92%
100-100%
113-122%
86-98%
EDTA plasma(n=5)
103-113%
90-101%
83-93%
108-118%
heparin plasma(n=5)
101-111%
113-123%
80-91%
86-97%
文献
1. Effect of ω-3 Fatty Acid on GastrointestinalMotility after Abdominal Operation in Rats