E-AXEAO-1KU: Supplied at ~ 1,000 U/mL. E-AXEAO-3KU: Supplied at ~ 1,000 U/mL.
Expression:
Recombinant from Orpinomyces sp.
Specificity:
Catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, α-napthyl acetate and 4-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin.
Specific Activity:
~ 36 U/mg (40oC, pH 6.7 on 4-nitrophenyl acetate)
Unit Definition:
One Unit of acetylxylan esterase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl acetate in sodium phosphate buffer (100 mM), pH 6.7 at 40oC.
Temperature Optima:
40oC
pH Optima:
7
Application examples:
Applications established in carbohydrate research and biofuel industries.