Catalyses the reaction: L-Asparagine + H2O = L-aspartate + NH3
Specific Activity:
~ 15 U/mg (25oC, pH 8.0 on L-asparagine); ~ 30 U/mg (37oC, pH 8.0 on L-asparagine)
Unit Definition:
One Unit of asparaginase activity is defined as the amount of enzyme required to produce one µmole of L-aspartate from L-asparagine (7.3 mM) per minute in the presence of NADPH in Tris.HCL buffer (45 mM), pH 8.0.
Temperature Optima:
37oC
pH Optima:
8
Application examples:
Applications established for the measurement of asparagine in the food, fermentation, wine, beverage, medical and pharmacological industries.