Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.1%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type
Average(%)
Recovery Range(%)
Serum
86
80-92
Plasma
88
82-94
线性
给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample
1:2
1:4
1:8
1:16
serum(n=5)
104-116%
101-110%
102-114%
113-123%
EDTA plasma(n=5)
81-93%
102-111%
90-102%
86-97%
heparin plasma(n=5)
103-113%
92-101%
98-109%
92-92%
文献
1. Regulation of the estrous cycle by neutrophils via opioid peptides