Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.4%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type
Average(%)
Recovery Range(%)
Serum
100
94-106
Plasma
102
96-108
线性
给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample
1:2
1:4
1:8
1:16
serum(n=5)
110-122%
109-120%
109-119%
114-124%
EDTA plasma(n=5)
104-114%
105-115%
91-103%
98-111%
heparin plasma(n=5)
88-97%
107-117%
100-112%
80-90%
文献
1. High disease activity in ankylosing spondylitis is associated with increased serum sclerostin level and decreased wingless protein-3a signaling but is not linked with greater structural damage
Auth: M Korkosz, J Gąsowski, P Leszczyński, et al.
From: BMC Musculoskeletal Disorders
2. Effect of tumour necrosis factor-α inhibitor on serum level of dickkopf-1 protein and bone morphogenetic protein-7 in ankylosing spondylitis patients