Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.9%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type
Average(%)
Recovery Range(%)
Serum
96
90-102
Plasma
98
92-104
线性
给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample
1:2
1:4
1:8
1:16
serum(n=5)
105-115%
87-98%
103-114%
110-120%
EDTA plasma(n=5)
99-110%
89-99%
89-101%
117-127%
heparin plasma(n=5)
88-88%
106-118%
93-101%
89-98%
文献
1. Role of YAP-related T cell imbalance and epidermal keratinocyte dysfunction in the pathogenesis of atopic dermatitis