Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤5.5%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type
Average(%)
Recovery Range(%)
Serum
106
100-112
Plasma
108
102-114
线性
给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample
1:2
1:4
1:8
1:16
serum(n=5)
102-112%
81-91%
111-120%
106-117%
EDTA plasma(n=5)
98-108%
98-108%
108-120%
88-100%
heparin plasma(n=5)
87-97%
80-88%
99-107%
94-94%
文献
1. IL-34 is associated with obesity, chronic inflammation, and insulin resistance
Auth: EJ Chang, SK Lee, YS Song, et al.
From: The Journal of Clinical Endocrinology & Metabolism