Milenia GenLine HybriDetect 试剂盒是一种简单,全新的核酸扩增快速检测方法,适用于任何PCR和恒温扩增的扩增产物快速检测。只需要在设计扩增时,设计一条FITC标记的探针,和生物素标记的引物便可使用该产品快速检测核酸扩增产物。 Milenia GenLine HybriDetect 试剂盒具有高灵敏度、高特异性的特点,而且不需要特殊的试剂和仪器设备,因此可建立起方便快捷的新型检测手段和检测体系,有助于您提升科研技术、扩大科研成果、拓展研究领域。 工作原理 Milenia GenLine HybriDetect 检测方法是结合了免疫技术、分子杂交技术、胶体金标记技术及侧向流层析技术的核酸快速检测试剂盒。其原理为生物素标记的PCR产物与FITC标记的特异探针杂交、FITC标记的特异探针与抗FITC抗体的金标物结合后,将该免疫复合物滴加到试纸条上,免疫复合物通过层析膜扩散,扩散到检测线时,生物素标记的扩增产物被生物素配体捕获,形成具有颜色的检测线。不被捕获的免疫复合物继续扩散到质控线被特异性抗体所捕获,形成具有颜色的质控线。 Milenia Biotec is dedicated to the development, manufacture and sales of point-of care lateral flow tests, a technology well known from pregnancy testing. The core products of the company are based on “universal test strips”, a concept allowing quick and simple developments and a simple production process. Milenia Biotec is located in Gießen, a university city with 84.000 inhabitants, 45% of whom are students. Gießen is in the center of Germany, 60 km north of Frankfurt/Main with its international airport. From here we serve our customers worldwide directly and via our network of partners and distributors.