HT-29/MTX /HT-29/MTX /HT-29/MT

HT-29/MTX /HT-29/MTX 细胞系/HT-29/MTX 细胞株/HT-29/MTX 人结肠癌细胞甲氨蝶呤细胞

Cell line name HT-29-MTX-E12

Synonyms HT29-MTX-E12; MTX-E12

Accession CVCL_G356

Resource Identification Initiative To cite this cell line use: HT-29-MTX-E12 (RRID:CVCL_G356)

Comments Population: Caucasian.

Selected for resistance to: ChEBI; CHEBI:44185; Methotrexate (MTX).

Derived from site: In situ; Colon; UBERON=UBERON_0001155.

Sequence variations

Mutation; HGNC; 583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).

Mutation; HGNC; 583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).

Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).

Mutation; HGNC; 8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).

Mutation; HGNC; 6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).

Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).

Disease Colon adenocarcinoma (NCIt: C4349)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Parent: CVCL_6834 (HT-29-MTX)

Sex of cell Female

Age at sampling 44Y

Category Cancer cell line

STR profile Source(s): ECACC=12040401

Markers:

Amelogenin X

CSF1PO 11,12

D5S818 11,12

D7S820 10

D13S317 11

D16S539 11,12

TH01 6

TPOX 8,9

vWA 17

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Publications

PubMed=11587485; DOI=10.1023/A:1010974909998

Behrens I., Stenberg P., Artursson P., Kissel T.

Transport of lipophilic drug molecules in a new mucus-secreting cell culture model based on HT29-MTX cells.

Pharm. Res. 18:1138-1145(2001)