THLE-2/THLE-2/THLE-2/人肝永生化细胞

THLE-2/THLE-2细胞系/THLE-2细胞株/THLE-2人肝永生化细胞

Cell line name    THLE-2
Synonyms    THLE2; Transformed Human Liver Epithelial-2
Accession    CVCL_3803
Resource Identification Initiative    To cite this cell line use: THLE-2 (RRID:CVCL_3803)
Comments    Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10149.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Cell type: Epithelial cell; CL=CL_0000066.
Species of origin    Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell    Male
Age at sampling    Adult
Category    Transformed cell line
STR profile    Source(s): AddexBio=T0015001/4912; ATCC=CRL-2706; IZSLER=BS CL 194

Markers:
Amelogenin    X,Y
CSF1PO    11,13
D2S1338    17,23
D3S1358    14,15
D5S818    11,13
D7S820    10,12
D8S1179    13
D13S317    8,12
D16S539    11,13
D18S51    13,16
D19S433    14
D21S11    30,32.2
FGA    22,24
Penta D    10,13
Penta E    12,13
TH01    7,9.3
TPOX    8,11
vWA    16,17

Run an STR similarity search on this cell line
Publications    
DOI=10.1007/978-1-4612-0411-4_31
Lechner J.F., Smoot D.T., Pfeifer A.M.A., Cole K.H., Weston A., Groopman J.D., Shields P.G., Tokiwa T., Harris C.C.
A non-tumorigenic human liver epithelial cell culture model for chemical and biological carcinogenesis investigations.
(In book chapter) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim J.S., Dritschilo A. (eds.); pp.307-321; Humana Press; New York; USA (1990)

PubMed=7685115; DOI=10.1073/pnas.90.11.5123; PMCID=PMC46667
Pfeifer A.M.A., Cole K.E., Smoot D.T., Weston A., Groopman J.D., Shields P.G., Vignaud J.-M., Juillerat M., Lipsky M.M., Trump B.F., Lechner J.F., Harris C.C.
Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.
Proc. Natl. Acad. Sci. U.S.A. 90:5123-5127(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Lehman T.A., Modali R.V., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

Patent=US5529920
Cole K.H., Lechner J.F., Reddel R.R., Harris C.C., Pfeifer A.M.A.
Human liver epithelial cell line and culture media therefor.
Patent number US5529920, 25-Jun-1996

Patent=US5759765
Harris C.C., Cole K.H., Lechner J.F., Reddel R.R.
Human liver epithelial cell lines.
Patent number US5759765, 02-Jun-1998

PubMed=23887712; DOI=10.1038/ncomms3218; PMCID=PMC3731665
Nault J.-C., Mallet M., Pilati C., Calderaro J., Bioulac-Sage P., Laurent C., Laurent A., Cherqui D., Balabaud C., Zucman-Rossi J.
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Nat. Commun. 4:2218.1-2218.7(2013)