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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
HT22
- 库存：
1x10^6/瓶/支
- 供应商：
上海酶研
- 肿瘤类型：
详询
- 细胞类型：
小鼠海马神经元细胞系
- ATCC Number：
详询
- 品系：
HT22
- 组织来源：
小鼠海马神经元细胞系
- 相关疾病：
HT22
- 物种来源：
哺乳动物
- 免疫类型：
详询
- 细胞形态：
贴壁/悬浮
- 是否是肿瘤细胞：
详询
- 器官来源：
小鼠海马神经元细胞系
- 运输方式：
顺丰快递
- 年限：
5年
- 生长状态：
生长良好
HT22/HT22细胞系/HT22细胞株/HT22小鼠海马神经元细胞系
Cell line name HT22
Synonyms HT-22
Accession CVCL_0321
Resource Identification Initiative To cite this cell line use: HT22 (RRID:CVCL_0321)
Comments Characteristics: Highly sensitive to glutamate and is therefore used to study glutamate-induced toxicity in neuronal cells.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Omics: Deep quantitative phosphoproteome analysis.
Omics: Secretome proteome analysis.
Derived from site: In situ; Brain, hippocampus; UBERON=UBERON_0002421.
Cell type: Neuron; CL=CL_0000540.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Hierarchy Parent: CVCL_U378 (HT4 [Mouse neurons])
Children:
CVCL_C0DX (HT-22 rho-0) CVCL_C0NE (HT22 Klf13 KO) CVCL_C0NF (HT22 Klf9 KO)
CVCL_C0UA (HT22 TR/TO-rKlf9 clone 2.1) CVCL_C0UB (HT22 TR/TO-mKlf13) CVCL_C0U8 (HT22-BLRP-hTRb/pSV40-BirAV5)
CVCL_C0U9 (HT22-FLBIO-rKLF9/SV40-BirAV5) CVCL_C0U7 (HT22-pSV40-BirAV5) CVCL_D6BT (HyCyte HT22 KO-mNlrp3)
CVCL_E2Q5 (HyCyte HT22 mSirt2_p.S368D_c.1102T>G_c.1103C>A) CVCL_D9WN (Ubigene HT22 Atg5 KO) CVCL_D9WP (Ubigene HT22 Atp7b KO)
CVCL_D9WQ (Ubigene HT22 Cbs KO) CVCL_D9WR (Ubigene HT22 Dgcr8 KO) CVCL_D9WS (Ubigene HT22 Lox KO)
CVCL_D9WT (Ubigene HT22 Prlr KO)
Category Transformed cell line
STR profile Source(s): Millipore=SCC129
Markers:
Mouse STR 1-1 10,11
Mouse STR 1-2 13,17
Mouse STR 2-1 9
Mouse STR 3-2 14,15
Mouse STR 4-2 18.3,19.3,20.3
Mouse STR 5-5 13,14,15
Mouse STR 6-4 15.3
Mouse STR 6-7 12
Mouse STR 7-1 29
Mouse STR 8-1 15
Mouse STR 11-2 15,17,18
Mouse STR 12-1 20
Mouse STR 13-1 16.2
Mouse STR 15-3 20.3
Mouse STR 17-2 12,13,14
Mouse STR 18-3 17,19
Mouse STR 19-2 11,12
Mouse STR X-1 25
Publications
PubMed=7953717; DOI=10.1016/0006-8993(94)90334-4
Davis J.B., Maher P.
Protein kinase C activation inhibits glutamate-induced cytotoxicity in a neuronal cell line.
Brain Res. 652:169-173(1994)
PubMed=9292733; DOI=10.1016/S0896-6273(00)80953-8
Li Y., Maher P., Schubert D.
A role for 12-lipoxygenase in nerve cell death caused by glutathione depletion.
Neuron 19:453-463(1997)
PubMed=19135458; DOI=10.1016/j.lfs.2008.12.008
Liu J., Li L.-X., Suo W.Z.
HT22 hippocampal neuronal cell line possesses functional cholinergic properties.
Life Sci. 84:267-271(2009)
PubMed=26227174; DOI=10.1002/pmic.201400623
Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.
In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.
Proteomics 15:3617-3622(2015)
PubMed=33430126; DOI=10.3390/nu13010179; PMCID=PMC7826960
Erber L.N., Luo A., Gong Y., Beeson M., Tu M.-L., Tran P.V., Chen Y.
Iron deficiency reprograms phosphorylation signaling and reduces O-GlcNAc pathways in neuronal cells.
Nutrients 13:179.1-179.19(2021)
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
a look from the following website just by clicking "Tyrosine Phosphorylation and Dimerization" http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-43F85YV-2&_user=1111158&_coverDate=06%2F29%2F2001&_rdoc=1&_fmt=full&_orig=search
扫描仪中，都采用机械式的二维X，Y线性扫描技术实现，即X，Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置，由于驱动系统的频率限制，驱动器的扫描惯性大，使得扫描效率低，分析时间相当长；并且往复行程长，对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描，我们的实验装置设计如图2，采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描，Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中，对于f-è物镜，满足x＝2fè（è为振镜的摆动角度，f为物镜焦距）的线性
Analysis of RB Action in DNA Damage Checkpoint Response
checkpoint response: (1) transcriptional repression of E2F-regulated genes (cyclin A reporter assay); (2) induction of cell cycle arrest (Brd-U incorporation assay); and (3) inhibition of DNA double-strand break accumulation (phosphorylated-histone H2A.X
技术资料
