NCI-H441 [H441] /NCI-H441 [H4

NCI-H441 [H441] /NCI-H441 [H441] 细胞系/NCI-H441 [H441] 细胞株/NCI-H441 [H441] 人肺腺癌细胞

Cell line name NCI-H441

Synonyms H441; H-441; NCI-H441-4; NCI-441; NCIH441

Accession CVCL_1561

Resource Identification Initiative To cite this cell line use: NCI-H441 (RRID:CVCL_1561)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: MD Anderson Cell Lines Project.

Part of: NCI RAS program mutant KRAS cell line panel.

Part of: RAS genetic alteration cell panel (ATCC TCP-1031).

Population: Caucasian.

Doubling time: 58 hours (Note=In RPMI 1640 + 10% FBS), 138 hours (Note=In ACL-3), 99 hours (Note=In ACL-3 + BSA) (PubMed=3940644); 80 hours (PubMed=25984343); 46.5 hours (PubMed=29681454).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: Metastatic; Pericardial effusion; UBERON=UBERON_0002409.

Cell type: Club cell; CL=CL_0000158.

Sequence variations

Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012583; Zygosity=Heterozygous (PubMed=1311061; ATCC=HTB-174; Cosmic-CLP=908460; DepMap=ACH-000638).

Mutation; HGNC; 11998; TP53; Simple; p.Arg158Leu (c.473G>T); ClinVar=VCV000528248; Zygosity=Homozygous (PubMed=1311061; PubMed=10536175; PubMed=20557307; Cosmic-CLP=908460; DepMap=ACH-000638).

HLA typing Source: PubMed=26589293

Class I

HLA-A A*02:01,03:01

HLA-B B*38:01,44:03

HLA-C C*16:02,16:02

Class II

HLA-DQ DQB1*06:07,06:07

HLA-DR DRB1*13:23,14:103

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 0.09

Native American 0

East Asian, North 3.45

East Asian, South 0

South Asian 1.1

European, North 61.67

European, South 33.69

Disease Lung papillary adenocarcinoma (NCIt: C5650)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_M254 (NCI-H441/CMV-Luc)

Sex of cell Male

Age at sampling 33Y

Category Cancer cell line

STR profile Source(s): AddexBio=C0016019/4975; ATCC=HTB-174; CCRID=4201HUM-CCTCC00308; CLS=305219; Cosmic-CLP=908460; PubMed=25877200

 

Markers:

Amelogenin X,Y

CSF1PO 11,12

D2S441 14

D2S1338 17,19

D3S1358 18

D5S818 11,12

D6S1043 17

D7S820 10

D8S1179 8,14

D12S391 19.3

D13S317 9

D16S539 9,13

D18S51 18,19

D19S433 14

D21S11 32.2

FGA 24,25

Penta D 10,12

Penta E 12

TH01 9.3

TPOX 8,10

vWA 17

 

Publications

PubMed=3940644

Brower M., Carney D.N., Oie H.K., Gazdar A.F., Minna J.D.

Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.

Cancer Res. 46:798-806(1986)

 

PubMed=2386953

Gazdar A.F., Linnoila R.I., Kurita Y., Oie H.K., Mulshine J.L., Clark J.C., Whitsett J.A.

Peripheral airway cell differentiation in human lung cancer cell lines.

Cancer Res. 50:5481-5487(1990)

 

PubMed=1311061

Mitsudomi T., Steinberg S.M., Nau M.M., Carbone D.P., D'Amico D., Bodner S.M., Oie H.K., Linnoila R.I., Mulshine J.L., Minna J.D., Gazdar A.F.

p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features.

Oncogene 7:171-180(1992)

 

PubMed=8806092; DOI=10.1002/jcb.240630505

Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.

NCI-Navy Medical Oncology Branch cell line data base.

J. Cell. Biochem. Suppl. 24:32-91(1996)

 

PubMed=10536175; DOI=10.3892/ijo.15.5.927

Fujita T., Kiyama M., Tomizawa Y., Kohno T., Yokota J.

Comprehensive analysis of p53 gene mutation characteristics in lung carcinoma with special reference to histological subtypes.

Int. J. Oncol. 15:927-934(1999)

 

PubMed=11005564; DOI=10.1038/sj.neo.7900094; PMCID=PMC1550293

Kohno T., Sato T., Takakura S., Takei K., Inoue K., Nishioka M., Yokota J.

Mutation and expression of the DCC gene in human lung cancer.

Neoplasia 2:300-305(2000)

 

PubMed=11030152; DOI=10.1038/sj.onc.1203815

Modi S., Kubo A., Oie H.K., Coxon A.B., Rehmatulla A., Kaye F.J.

Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.

Oncogene 19:4632-4639(2000)

 

PubMed=18083107; DOI=10.1016/j.cell.2007.11.025

Rikova K., Guo A.-L., Zeng Q.-F., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y.-R., Tan Z.-P., Stokes M.P., Sullivan L., Mitchell J., Wetzel R., MacNeill J., Ren J.-M., Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D.-Q., Zhou X.-M., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.

Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.

Cell 131:1190-1203(2007)

 

PubMed=19153074; DOI=10.1093/hmg/ddp034

Medina P.P., Castillo S.D., Blanco S., Sanz-Garcia M., Largo C., Alvarez S., Yokota J., Gonzalez-Neira A., Benitez J., Clevers H.C., Cigudosa J.C., Lazo P.A., Sanchez-Cespedes M.

The SRY-HMG box gene, SOX4, is a target of gene amplification at chromosome 6p in lung cancer.

Hum. Mol. Genet. 18:1343-1352(2009)

 

PubMed=19472407; DOI=10.1002/humu.21028; PMCID=PMC2900846

Blanco R., Iwakawa R., Tang M.-Y., Kohno T., Angulo B., Pio R., Montuenga L.M., Minna J.D., Yokota J., Sanchez-Cespedes M.

A gene-alteration profile of human lung cancer cell lines.

Hum. Mutat. 30:1199-1206(2009)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

 

PubMed=20557307; DOI=10.1111/j.1349-7006.2010.01622.x; PMCID=PMC11158680

Iwakawa R., Kohno T., Enari M., Kiyono T., Yokota J.

Prevalence of human papillomavirus 16/18/33 infection and p53 mutation in lung adenocarcinoma.

Cancer Sci. 101:1891-1896(2010)