Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.2%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。
Sample Type
Average(%)
Recovery Range(%)
Serum
94
88-100
Plasma
96
90-102
线性
给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。
Sample
1:2
1:4
1:8
1:16
serum(n=5)
97-107%
112-121%
112-122%
98-107%
EDTA plasma(n=5)
98-110%
108-118%
91-104%
80-90%
heparin plasma(n=5)
92-102%
98-110%
95-104%
82-92%
文献
1. Serum activated leukocyte cell adhesion molecule and intercellular adhesion molecule-1 in patients with gastric cancer:Can they be used as biomarkers?
Serum activated leukocyte cell adhesion molecule and intercellular adhesion molecule-1 in patients with gastric cancer: Can they be used as biomarkers?
Kayhan Erturka,*, Didem Tastekina, Elif Bilginb, Murat Serilmezb, Hamza Ugur Bozbeya,Burak Sakara