- 询价
- Biotium
- 美国
- 40060-T
- 2026年01月20日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
1年
- 英文名：
RedDot 1, 200X 40060-T
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
RedDot™1 is a far-red cell membrane-permeant nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells.
- Ideal for specifically staining the nuclei of live cells for short term imaging
- Highly thermostable and photostable, for convenient handling and demanding imaging applications
- Can be used for DNA content analysis by flow cytometry like Vybrant™ DyeCycle™ Ruby
- Can be used for normalization of In Cell Western® in fixed cells, similar to Draq5™/Sapphire®700
- λEx/λEm = 662/694 nm (with DNA), for detection in the Cy®5 channel
RedDot™1 can be excited at a wide range of wavelengths between 488 nm and 647 nm, including the 488 nm laser for flow cytometry, and can be used for DNA content analysis by flow cytometry. While DAPI and Hoechst dyes show a strong preference for A-T rich regions, RedDot™1 is relatively insensitive to sequence base composition.
Choose the Right Dye for Your Application
Like Draq5®, RedDot™1 is toxic to cells within a few hours, and is recommended for short term staining experiments. For a low-toxicity far-red nuclear stain for live cells, see our NucSpot™ 650 dye.
RedDot™1 can be used to stain live bacteria (gram-positive and gram-negative). Staining in live yeast is weak and not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.
RedDot™1 cannot be used for nuclear-specific staining in fixed cells. Please see RedDot™2 (cat# 40061), a spectrally similar dye designed for specific nuclear staining of fixed and permeabilized cells or selective nuclear staining of dead cells.
Please note: far-red dyes like RedDot™1 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.
RedDot™1 and RedDot™2 Far-Red Nuclear Stains
| Product | Catalog No. | Cell Permeability | Live/Fixed | Color (Ex/Em) | Applications |
|---|---|---|---|---|---|
| RedDot™1 Far-Red Nuclear Stain, 200X in Water | 40060 | Permeant | Live cells only | (Far-red) 662/694 nm |
• Live cell nuclear stain comparable to Draq5™ • For microscopy, cell cycle analysis by flow cytometry • Can be used for In-Cell Western normalization |
| RedDot™2 Far-Red Nuclear Stain, 200X in DMSO | 40061 | Impermeant | Fixed/permeabilized cells or tissues | (Far-red) 665/695 nm |
• Fixed cell nuclear stain • Greater nuclear specificity than Draq7™ • Selectively stains dead cells in unfixed cultures |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Tamoxifen administration to mice
dissolve 100mg tamoxifen in 100µl pure ethanol (10mg/100µl = 100mg/ml tamoxifen) dilute 100-200x in distilled water to final concentration of 1-0.5mg/ml use 1% ethanol in distilled water as a negative control replace water in drinking
Purification of recombinant sBRF M166L
of beads will not be very firm so it seems best to remove as much supernatant as possible with a pipette and don’t worry if not all the sup. can be removed). Save this supernatant in case there is a problem with the BRF binding. Resuspend the beads
20x T.E. 0.5 µL RiboGreen (200x) 92.5 µL DEPC treated H2 O 2. Aliquot 98.5 µL master mix into 1.5 mL tubes. 3. Setup samples and controls: Add 2 µL H2 O into one tube as the "blank". Add 2 µL of RNA
