- 询价
- Biotium
- 美国
- 40054
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
dNTP Mix, 10mM each 40054
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
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文献和实验ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
O (q.s. 10 ul) Mix and zing. Heat to 80°C, 10min, then immediately ice In separate mfuge tube on ice mix: ("Reaction Mix" 10ul) 4ul 5X RT buffer (Vial 5) 1ul dNTP mix (Vial 4) 4ul RF H20 1ul (200 Units) reverse transcriptase enzyme (Vial
Linear amplification of limiting amounts of RNA for gene expression studies
-dT-T7-primer (500ng/µl), incubate the mix at 70°C for 10 minutes, snap-cool on ice; Prepare the following reverse transcription mix: 4µl 5x First strand cDNA buffer, 2µl 0.1M DTT, 1µl 10mM dNTP mix, 1µl RNAsin, 2µl Superscript II RT;
Amplification and Labelling with Cy dyes
mix 1 (for 2 reactions): 2ml 5X T7 Sequenase buffer 3ml 10mM dNTP mix 1.5ml 0.1M DTT 3ml 500mg/ml BSA 0.6ml 13U/ml T7 Sequenase Reaction mix 2 (for 2 reactions): 1.4ml Sequenase dilution buffer 0.6ml 13U/ml T7 Sequenase Program themalcycler
