- ￥800
- 华纳生物
- WN-12981
- 武汉
- 2025年07月14日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 库存：
咨询
- 英文名：
pDsRed2-Mito
- CAS号：
咨询
- 保质期：
咨询
- 供应商：
武汉华尔纳生物科技有限公司
- 保存条件：
咨询
Product Introduction
| 品牌 |
华纳生物 | ||||||||||||||||||||
| 货号 |
WN-12981 | ||||||||||||||||||||
| 规格 |
2ug | ||||||||||||||||||||
| 价格 |
询价 | ||||||||||||||||||||
| 货期 |
现货 | ||||||||||||||||||||
订购信息
质粒图谱 载体描述pDsRed2-Mito is a mammalian expression vector that encodes a fusion of Discosoma sp. red fluorescent protein (DsRed2; 1, 2) and the mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (Mito; 3, 4). The Mito sequence is fused to the 5'-end of DsRed2, a human codon-optimized DsRed variant that is engineered for faster maturation and lower nonspecific aggregation (1, 5). The Mito sequence targets the Mito-DsRed2 fusion protein to the host cell’s mitochondria. To drive expression of Mito-DsRed2, this vector contains the immmediate early promoter of cytomegalovirus (PCMV IE ). SV40 polyadenylation signals downstream of the DsRed2 gene direct proper processing of the 3'-end of the Mito-DsRed2 mRNA. This vector also contains an SV40 origin for replication in any mammalian cell line that expresses the SV40 T-antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassette—consisting of the SV40 early promoter (PSV40e), the neomycin/kanamycin resistance gene of Tn5 (Neor/Kanr), and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV TK poly A) gene—allow stably transfected eukaryotic cells to be selected using G418 (6). A bacterial promoter (P) upstream of this cassette drives expression of the gene encoding kanamycin resistance in E. coli. 用途pDsRed2-Mito is designed for fluorescent labeling of mitochondria. The vector can be introduced into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (6). The Mito-DsRed2 fusion (excitation/emission maxima: 558 nm/ 583 nm) can be detected by fluorescence microscopy and by flow cytometry. Filter sets optimized for detecting DsRed by microscopy are available from Chroma Technology Corporation and Omega Optical Inc. Please see their websites (www.chroma.com and www.omegafilters.com) and the Living Colors® Vol. II User Manual, provided with this vector, for more information. To detect Mito- DsRed2-expressing cells by flow cytometry, use the instrument’s argon-ion laser to excite the fluorophore at 488 nm and the FL-2 channel to detect the fluorophore’s emission at 583 nm. |
|||||||||||||||||||||
武汉华尔纳生物科技有限公司，简称华尔纳生物（Warner Bio），坐 落于武汉生物产业基地·光谷生物城，是一家生物医学资源开发，科研生 产，产品销售集一体的科技型企业。
华尔纳生物（Warner Bio）始终专注于生命科学领域及细胞实验培养 方向，坚持为客户打造优质高效，安全可靠的生命科学产品，我们始终坚 持于以客户为中心、以品质求生存、以价值求发展、以服务求口碑的企业 理念，从而获得全国广大科研用户的一致认可。
团队以高效的服务品质，专业卓越的技术能力构建了华中地区最大的 生物细胞资源典藏中心，经过多年不懈的努力与发展，现与武汉大学、华 中科技大学、武汉协和医院、武汉同济医院、四川大学华西医院、首都医 科大学、中山大学附属第三医院等全国多所高校、医院、研究所单位达成 长期稳定的合作，同时为科研人员提供免费专业的售前售中售后服务，为 您的科研实验保驾护航。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
