- ￥800
- 华纳生物
- WN-12970
- 武汉
- 2025年07月15日
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- 详细信息
- 技术资料
- 库存：
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- 英文名：
pEF1/myc-His A
- CAS号：
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- 保质期：
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- 供应商：
武汉华尔纳生物科技有限公司
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Product Introduction
| 品牌 |
华纳生物 | ||||||||||||||||||||
| 货号 |
WN-12970 | ||||||||||||||||||||
| 规格 |
2ug | ||||||||||||||||||||
| 价格 |
询价 | ||||||||||||||||||||
| 货期 |
现货 | ||||||||||||||||||||
订购信息
质粒图谱 载体描述pEcoli-6xHN-GFPuv is a control vector that encodes a GFPuv fusion protein containing an N-terminal 6xHN tag (composed of 6 repeating His-Asn subunits) and an enterokinase cleavage (EK) site. High-level, IPTG-inducible expression of the fusion is driven by the T7 lac hybrid promoter (PT7 lac), which consists of the strong T7 promoter (1–3) and the lac operator (4). Basal expression of the fusion is repressed by the Lac repressor (lacI), which binds to the lac operator, preventing expression from the promoter in the absence of IPTG. High-level, IPTG-inducible expression of 6xHN-tagged GFPuv is driven by the T7 promoter in the presence of T7 RNA polymerase. The vector also contains an ampicillin resistance gene (Ampr) and a pBR322 origin of replication, which maintains the vector at a low-copy-number to further reduce basal levels of the fusion. 载体应用pEcoli-6xHN-GFPuv is included with Clontech’s pEcoli Expression System and pEcoli Linear Expression System (Cat. Nos. 631417 & 631418, respectively). GFPuv is a green fluorescent protein variant optimized for maximal fluorescence when excited by UV light. The vector allows the use of GFPuv fluorescence (excitation and emission maxima at 395 nm and 509 nm, respectively) to monitor protein expression and purification. His-tagged proteins, including 6xHN-GFPuv, can be readily detected with either our Universal His Western Blot Kit (Cat No. 635633) or our 6xHN Polyclonal Antibody (631207). Exceptionally pure his-tagged proteins can be obtained with our TALON Co resins (Cat. Nos. 635501–635507, 635509 & 635510) and columns (Cat. Nos. 635601–635603 and 635606). For routine use, we have a variety of high capacity His60 Ni resins available (Cat. Nos. 635659–635664). In addition, an assortment of starter kits containing your choice of TALON Co or His60 Ni resin, as well as pEcoli-Nterm/-Cterm 6xHN circular or linear vectors (Cat Nos. 631416, and 631419–631427) are also available. |
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武汉华尔纳生物科技有限公司，简称华尔纳生物（Warner Bio），坐 落于武汉生物产业基地·光谷生物城，是一家生物医学资源开发，科研生 产，产品销售集一体的科技型企业。
华尔纳生物（Warner Bio）始终专注于生命科学领域及细胞实验培养 方向，坚持为客户打造优质高效，安全可靠的生命科学产品，我们始终坚 持于以客户为中心、以品质求生存、以价值求发展、以服务求口碑的企业 理念，从而获得全国广大科研用户的一致认可。
团队以高效的服务品质，专业卓越的技术能力构建了华中地区最大的 生物细胞资源典藏中心，经过多年不懈的努力与发展，现与武汉大学、华 中科技大学、武汉协和医院、武汉同济医院、四川大学华西医院、首都医 科大学、中山大学附属第三医院等全国多所高校、医院、研究所单位达成 长期稳定的合作，同时为科研人员提供免费专业的售前售中售后服务，为 您的科研实验保驾护航。
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