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文献和实验Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley
Immunoprecipitation and Immune Complex kinase assay--according to Tamara Hurley 1) Wash cells with cold "Tris" (from Margie's shelf). Spin down the cells if you are working with suspension cells in a low speed swinging
RNA Purification Protocol for 1-2 x 107 cultured cells
设备 1. Microcentrifuge capable of 3,000 x g 2. 15 ml centrifuge tube 3. Ice bath 4. Ground pestle 实验步骤 1. Remove the media and collect cells. For cells grown in suspension, pellet the cell
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
centrifugation (3000-5000 x g (6600 rpm) for 1 min)), and remove supernatant (use gel loading tips to completely remove supernatant). (23) Add 1 ml of ice-cold Low Salt Immune Complex Wash Buffer (EZ ChIP kit). Resuspend the packed agarose beads by gentle
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