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文献和实验实验步骤 1. Protocol for FFPE Extraction 1) cut a approximately 10 mg FFPE sample into a 2 ml collection tube or suitable vessel, cut off the Samples tiny and remove the paraffin wax as far as possible.
Bisulfite Conversion Based PCR
The bisulfite reaction reaction was first described in early 1970s and was used by Frommer et al to distinguish between cytosine and 5mCytosine (5mC) in DNA. In this reaction, DNA is first treated with sodium bisulfite to convert cytosine
DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones
DNA methylation is an essential epigenetic modification in the human genome. For the investigation of DNA methylation patterns, bisulfite conversion and DNA sequencing is a method of choice, because it provides detailed information
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