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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG1
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite®594 Fluorescent Dye
- 适应物种:
human, mouse, rat, pig
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
IF-P, FC (Intra)
- 靶点:
Alpha smooth muscle actin specific
- 抗体英文名:
CoraLite®594-conjugated Alpha smooth muscle actin specific Monoclonal antibody
- 抗体名:
CoraLite®594-conjugated Alpha smooth muscle actin specific Monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67735 targets Alpha smooth muscle actin specific in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P, FC (Intra) |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | actin, alpha 2, smooth muscle, aorta |
| 别名 | ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | NM_001613 |
| 基因名称 | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_2920177 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P62736 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
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文献和实验| Species | Application | Title |
|---|---|---|
| IF | Cell Rep Med A functional cardiac patch promotes cardiac repair by modulating the CCR2- cardiac-resident macrophage niche and their cell crosstalkAuthors - Chengbin Ding |
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