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- 详细信息
- 技术资料
- 免疫原:
Recombinant human SOX2 protein
- 亚型:
IgG1
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite®594 Fluorescent Dye
- 适应物种:
human, mouse, rat
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
IF/ICC, IF-P, FC (Intra)
- 靶点:
SOX2
- 抗体英文名:
CoraLite®594-conjugated SOX2 Monoclonal antibody
- 抗体名:
CoraLite®594-conjugated SOX2 Monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF-P detected in | mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | U-251 cells |
| Positive FC (Intra) detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66411 targets SOX2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, IF-P, FC (Intra) |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag13635 Product name: Recombinant human SOX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-317 aa of BC013923 Sequence: MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 2 |
| 别名 | Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34-40 kDa |
| GenBank蛋白编号 | BC013923 |
| 基因名称 | SOX2 |
| Gene ID (NCBI) | 6657 |
| RRID | AB_2883578 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P48431 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
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