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- Proteintech
- CL594-60183
- 2025年10月13日
- IF/ICC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KRT20 protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite®594 Fluorescent Dye
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IF/ICC
- 靶点：
Cytokeratin 20
- 抗体英文名：
CoraLite®594-conjugated Cytokeratin 20 Monoclonal antibody
- 抗体名：
CoraLite®594-conjugated Cytokeratin 20 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HT-29 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60183 targets Cytokeratin 20 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag11336 Product name: Recombinant human KRT20 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 74-424 aa of BC031559 Sequence: MQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | keratin 20 |
| 别名 | Cell and organelle markers, CK 20, CK20, Cytokeratin 20, Cytoskeleton Marker, K20, keratin 20, KRT20, KRT21, Protein IT |
| 计算分子量 | 424 aa, 48 kDa |
| GenBank蛋白编号 | BC031559 |
| 基因名称 | Cytokeratin 20 |
| Gene ID (NCBI) | 54474 |
| RRID | AB_2919900 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35900 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18).Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
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文献和实验. P. MicroRNA-directed cleavage of HOXB8 mRNA. Science 304, 594–596 (2004) | Article | PubMed | ChemPort | 19. Damha, M. J. & Ogilvie, K. K. Oligoribonucleotide synthesis. The silyl-phosphoramidite method. Methods Mol. Biol. 20, 81–114 (1993) | PubMed
Disulfide Conjugation of Peptides to Oligonucleotides and Their Analogs
Thiol modifier C6‐S‐S (for 5′‐thiol modification, Glen Research) 0.02 M iodine solution in 78:2:20 (v/v/v) THF/pyridine/water (Proligo)
各种细胞中所含中间丝提纯制成特异性的单克隆抗体血清用于细胞学归属和病理学的检测。这些中间丝包括：①上皮细胞或上皮源细胞中的前角质蛋白（Prekeratin）或称细胞角质蛋白（cytokeratin）；②心肌、平滑肌或骨骼肌细胞中的结蛋白（Desmin）；③间质细胞或间充质
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