- ￥2800
- Proteintech
- CL488-67051
- 2025年10月13日
- IF/ICC, IF-P
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human IL-4R protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IF/ICC, IF-P
- 靶点：
IL-4RA/CD124
- 抗体英文名：
CoraLite® Plus 488-conjugated IL-4RA/CD124 Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated IL-4RA/CD124 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | Daudi cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67051 targets IL-4RA/CD124 in IF/ICC, IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, IF-P |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag28411 Product name: Recombinant human IL-4R protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-232 aa of NM_000418 Sequence: MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQH |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 4 receptor |
| 别名 | IL4R, 1E4C11, CD124, IL 4 binding protein, IL 4 receptor subunit alpha |
| 计算分子量 | 90 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | NM_000418 |
| 基因名称 | IL-4R |
| Gene ID (NCBI) | 3566 |
| RRID | AB_2919413 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P24394 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.
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文献和实验。 4、可订制的抗体包括：CD2，CD3，CD4，CD5，CD8，CD10，CD11b，CD14，CD16，CD19，CD20，CD24，CD25，CD27，CD29，CD33，CD34，CD36，CD38，CD41，CD45，CD45RA，CD56，CD66b，CD66e，CD69，CD124，HLA－DR， IgE， breast carcinoma, TCRαβ, TCRγδ RosetteSep® 回收率： 无FicollTM 离心后分选步骤对细胞的进一步丢失。 使用FicollTM 离心
Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
：单克隆抗体设立相应亚型的对照，而多克隆抗体需要对应种属的 IgG 作为对照。用血球计数法或是其他方法对细胞计数。 1. 将 0.5-1 x 106 个细胞分到各检测管中（依照体积）。 2. 各管加入 2-3 ml 孵育缓冲液漂洗，离心回收细胞。重复一次。 3. 将每个管中的细胞重悬在 100 µl 孵育缓冲液中。 4. 室温，孵育缓冲液封闭 10 分钟。 5. 按合适的稀释比例在检测管中加入未标记，生物素标记或是荧光素标记的一抗。具体的用量参考抗体各自的说明书。
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